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Listening Quiz: Tối Ưu Hóa PCR Cho Bộ Mồi Mới – Easy Level
Th7 31, 2024
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bởi
Anna Phạm
trong
Tiếng Anh dành cho Nhà sinh học (English for Biologist)
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1.
What is the main purpose of the conversation?
To decide which team member will lead the laboratory
To discuss how to design a new primer set from scratch
To plan how to optimize PCR conditions for a new primer set
To report the final results of a completed PCR experiment
Explanation:
The team is discussing how to optimize PCR conditions for a new primer set, including temperature, magnesium levels, and cycle number.
2.
Which PCR condition does Mark say they need to check first?
The primer length
The number of samples
The reaction volume
The annealing temperature
Explanation:
Mark says, ‘We need to check the annealing temperature.’
3.
What method will they use to find the right annealing temperature?
A gradient PCR with different temperatures
A repeated washing step
A standard curve
A DNA sequencing test
Explanation:
Mark explains that they will ‘run a gradient PCR with different temperatures.’
4.
How many PCR cycles does Mark say are usually enough?
30 to 35 cycles
40 to 45 cycles
20 to 25 cycles
10 to 15 cycles
Explanation:
Mark says, ‘Usually 30 to 35 cycles are enough.’
5.
What will the team do next afternoon?
Change the primer set and start over
Review the final data from the experiment
Publish the PCR protocol for the lab
Begin the tests and prepare the samples
Explanation:
Mark says, ‘We begin this afternoon. Please prepare the samples,’ which shows they will start the tests and prepare the samples.
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